Definitions for cosmid
cos·mid

Princeton's WordNetRate this definition:0.0 / 0 votes

1. cosmidnoun

   (genetics) a large vector that is made from a bacteriophage and used to clone genes or gene fragments

WiktionaryRate this definition:0.0 / 0 votes

1. cosmidnoun

   A type of plasmid (often used as a cloning vector) constructed by the insertion of cos sequences, which are DNA sequences of the lambda phage.

WikipediaRate this definition:0.0 / 0 votes

1. Cosmid

   A cosmid is a type of hybrid plasmid that contains a Lambda phage cos sequence. They are often used as a cloning vector in genetic engineering. Cosmids can be used to build genomic libraries. They were first described by Collins and Hohn in 1978. Cosmids can contain 37 to 52 (normally 45) kb of DNA, limits based on the normal bacteriophage packaging size. They can replicate as plasmids if they have a suitable origin of replication (ori): for example SV40 ori in mammalian cells, ColE1 ori for double-stranded DNA replication, or f1 ori for single-stranded DNA replication in prokaryotes. They frequently also contain a gene for selection such as antibiotic resistance, so that the transformed cells can be identified by plating on a medium containing the antibiotic. Those cells which did not take up the cosmid would be unable to grow.Unlike plasmids, they can also be packaged in vitro into phage capsids, a step which requires cohesive ends, also known as cos sites also used in cloning with a lambda phage as a vector, however nearly all the lambda genes have been deleted with the exception of the cos sequence. The hybrid cosmid DNA in the capsids can then be transferred into bacterial cells by transduction. Since there is a requirement for in vitro packaging whereby at least 38 kb of DNA is required between the cos sites, the vector without insert DNA will not be packaged (plasmids instability is increased if the novel inserted DNA contains many direct repeats or palindromic (inverted repeats) DNA. This instability can largely be counteracted by using a host bacterium with specific mutations affecting DNA recombination (N.B. Absence of inverted repeats was noted in the first Hohn & Collins publication cited above; see also).

ChatGPTRate this definition:0.0 / 0 votes

1. cosmid

   A cosmid is a type of hybrid plasmid (a small, circular piece of DNA) that contains a lambda phage cos site, which allows it to be packaged into a virus particle in bacteria. Cosmids are used as a means of cloning large fragments of DNA, typically between 20 and 45 kilobases, into bacteria for genetic research. These bacterial vectors are especially useful in the creation of genomic libraries.

WikidataRate this definition:0.0 / 0 votes

1. Cosmid

   A cosmid, first described by Collins and Hohn in 1978, is a type of hybrid plasmid that contains a Lambda phage cos sequence. Cosmids' DNA sequences are originally from the lambda phage. Cosmids can be used to build genomic libraries.

Matched Categories

1. • Genetics
   • Vector

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Numerology

1. Chaldean Numerology

   The numerical value of cosmid in Chaldean Numerology is: 4

2. Pythagorean Numerology

   The numerical value of cosmid in Pythagorean Numerology is: 9

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References

1. ^ Princeton's WordNet
   http://wordnetweb.princeton.edu/perl/webwn?s=cosmid
2. ^ Wiktionary
   https://en.wiktionary.org/wiki/Cosmid
3. ^ Wikipedia
   https://en.wikipedia.org/wiki/Cosmid
4. ^ ChatGPT
   https://chat.openai.com
5. ^ Wikidata
   https://www.wikidata.org/w/index.php?search=cosmid